Table IV.
Effect of ROI scavengers and related inhibitors on ·OH release by radish seed coats
| Inhibitor | ·OH Release |
|---|---|
| % | |
| None | 100 ± 5 |
| Catalase (100 μg mL−1) | 45 ± 15 |
| KI (10 mm) | 7 ± 21 |
| Superoxide dismutase (100 μg mL−1) | 30 ± 18 |
| Mn-desferal (300 μm) | 26 ± 29 |
| KCN (1 mm) | −9 ± 23 |
| NaN3 (1 mm) | −14 ± 10 |
| Adenine (10 mm) | 26 ± 10 |
| Mannitol (50 mm) | 54 ± 22 |
| Mannitol (100 mm) | 8 ± 35 |
| Na-formate (10 mm) | 81 ± 8 |
| Na-formate (20 mm) | −35 ± 27 |
| Thiourea (50 mm) | 19 ± 14 |
| Urea (100 mm) | 83 ± 27 |
The ·OH-dependent hydroxylation of benzoate by 10 seed coats from 48-h germinated seeds during a 3-h incubation period was determined in the presence of various inhibitors. Urea served as a negative control.