Preparation of PEM buffer:
| Reagent | Final concentration | Amount |
|---|---|---|
| PIPES | 0.21 M | 63.5 g |
| EGTA | 1 mM | 0.38 g |
| MgSO4 | 2 mM | 0.24 g |
| Adjust pH-7.0 | n/a | Variable |
| ddH2O | n/a | Variable |
| Total | n/a | 1,000 mL |
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