REAGENT OR RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-GFP (1:500 dilution)	Aves labs	Cat# GFP-1020 ; RRID: AB_10000240
TO-PRO3 (1:500 dilution)	Thermo Fisher Scientific	Cat# T3605
anti-chicken secondary antibody	Molecular probes	Cat# A-11040; RRID: AB_2534097
Chemicals, peptides, and recombinant proteins
1-Phenyl-2-thiourea (PTU)	Acros organics	Cat# 207250250
Methylene blue	Fisher Scientific	Cat# M291-25
Tricaine-S	Syndel	Cat# 200-266
Instant Ocean Sea Salt	Instant Ocean	Cat# SS15-10
Formaldehyde	Fisher Scientific	Cat# 04042-500
Bovine serum Albumin (BSA)	Sigma-Aldrich	Cat# A8806
Phosphate Buffered Saline (pH, 7.4)	Thermo Fisher Scientific	Cat# 10010023
Dimethyl sulfoxide (DMSO)	Fisher Scientific	Cat# D128-500
Triton-X100	Sigma-Aldrich	Cat# T8787
PIPES	Sigma-Aldrich	Cat# P6757-500
EGTA	Fisher Scientific	Cat# 02783-100
MgSO4	Fisher Scientific	Cat# BP213-1
Tween-20	Sigma-Aldrich	Cat# P7949
Vectashield	Vector Laboratories, Inc.	Cat# H1000
Experimental models: Organisms/strains
Tg(mpeg:GFP) zebrafish	Zebrafish International Resource Center	Cat# ZL9940
Other
Fluorescence microscope	Olympus	Model :SZX2-ILLTS
Confocal Microscope	Nikon	Model: Eclipse Ti2
Corning™ Untreated Culture Dishes	Fisher Scientific	Cat# 08-772-32
Disposable safety scalpels (Curved)	Fine Science Tools	Cat# 10000-15