Fig. 2.

The Tagln⁺ stromal domain encompassed the majority of subepithelial Wnt producers. (A) Uniform manifold approximation and projection (UMAP) of 8767 mouse ileal stromal cells resulted in 22 distinct clusters. Wnt-expressing clusters are circled on the UMAP plot. (B) Violin plots showed the stromal domains expressing Wnt ligands and key stromal cell markers (Foxl1, Gli1, Pdgfrα, Cd81 and Grem1) across the 22 clusters. Tagln and Acta2 were highly expressed in the major Wnt2b-, Wnt4- and Wnt5a-expressing clusters. (C) UMAP plots for clusters 5, 9, 11, 14 and 21 showed a containment of these Wnt-producing cells by Tagln and Acta2. (D) Differential expression of Tagln, Acta2, Foxl1, Gli1, Pdgfrα, Grem1 and Cd81 in Wnt2b⁻ and Wnt2b⁺ cells, Wnt4⁻ and Wnt4⁺ cells, and Wnt5a⁻ and Wnt5a⁺ cells. (E) t-SNE plot and Venn diagram highlighted Tagln⁺/Foxl1⁻, Tagln⁺/Foxl1⁺ and Tagln⁻/Foxl1⁺ populations with exact numbers of cells in each population. Sixteen out of 235 Foxl1⁺ cells did not express Tagln. (F) Violin plots showed a similar transcriptomic expression between Tagln⁺/Foxl1⁺ and Tagln⁻/Foxl1⁺ populations, for Wnt, R-Spondin, DKK, Fgf, Sfrp1 and Grem molecules. Also see Fig. S2.