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. 2022 Jan 12;149(1):dev199587. doi: 10.1242/dev.199587

Fig. 4.

Fig. 4.

Wnt deprivation from epithelium and Tagln+ or Acta2+ cells diminished Reg4 cell differentiation in colon. (A) Histology analysis did not show differences in Gpr177L/−;Vil-Cre;Tagln-Cre and Gpr177L/−;Vil-Cre;Acta2-CreER mouse small intestines. (B-F) Gpr177L/−;Vil-Cre;Tagln-Cre intestines had reduced Olfm4+ (C), Lyz1+ (D) and EdU+ (E) cell numbers but unchanged numbers of nuclear β-catenin+ cells (F) (red arrowheads). Gpr177L/−;Vil-Cre;Acta2-CreER mice showed no detectable difference in the above analysis. n=3 mice per genotype. Fifty crypts of the small intestine were counted per marker per genotype. (G-I) Histology (G) and immunostaining for Reg4 and Muc2 (H) showed diminished and significantly reduced DCS cells (H) in Gpr177L/−;Vil-Cre;Tagln-Cre and Gpr177L/−;Vil-Cre;Acta2-CreER mouse colons, respectively. Goblet cells were not changed. n=3 mice per genotype. (J,K) Quantitative RT-PCR detected a reduction in Mmp7 in Gpr177L/−;Vil-Cre;Tagln-Cre intestines and a reduction in Defa5 in Gpr177L/−;Vil-Cre;Acta2-CreER intestines. n=3 mice per genotype. Data are mean±s.e.m. from at least three independent experiments. *P<0.05. Also see Fig. S4.