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. Author manuscript; available in PMC: 2022 Feb 26.
Published in final edited form as: J Vis Exp. 2019 Jan 25;(143):10.3791/58971. doi: 10.3791/58971

Figure 1: Schematic of the comet assay.

Figure 1:

(A) Mix the cell suspension with low-melting-point agarose and (B) place it on a glass slide. (C) Treat it with cell lysis buffer, followed by an alkaline solution, to get nucleoids containing supercoiled DNA. (D) Electrophorese and stain the DNA using SYBR green DNA dye. (E) Schematic of intact (left) and damaged DNA (right, comet shape). (F) Formula to calculate a fraction of the DNA damage and tail moment.