Fig. 3.

Rv0572c-specific IgG1, IgG2a, and total IgG antibodies in the immunized mice. Nine weeks after immunization, sera were collected from each C57BL/6 mouse in all groups (n = 6). The titers of rRv0572c-specific antibodies, total IgG, and subclasses in the sera were determined using ELISA. The results are shown as the mean (±SEM) (A) log₁₀ endpoint titer and (B) ratio of IgG2a/IgG1 in the immunized mice. Comparable results were obtained from two independent experiments. *P < 0.05, **P < 0.01.