Fig. 1.

Electrophoresis results of the amplicons derived from nested 5′ RACE and the housekeeping gene amplification. A Electrophoresis of nested 5′ RACE amplicons corresponding to the 5′ UTR of the human BCL2L12 gene, using the three distinct TSOs described in the present study. Each TSO was incorporated both in a typical 1-step and a custom designed 2-step RT reactions before the implementation of the nested 5′ RACE. B Electrophoresis of the amplicons corresponding to the GAPDH mRNA amplification, which was used as housekeeping for the present work