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. Author manuscript; available in PMC: 2022 Feb 26.
Published in final edited form as: Immunohorizons. 2021 Aug 30;5(8):721–732. doi: 10.4049/immunohorizons.2100071

Figure 2. pDCs are required for optimal T cell cytokine production in the gut-draining lymph node throughout S. mansoni infection.

Figure 2.

WT or BDCA-2-DTR (DTR) mice were infected with ~40–80 S. mansoni (Sm) cercariae on d0 (naïve (N)). For assessment of pDCs in acute infection from d32 until d42 (a), or for assessment at the onset of chronic infection, from d42 until d56 (e), mice were treated i.p. with DTx before MLN harvest. (b, f) Proportion and total numbers of pDCs from MLN cells on d42 (b) or d56 (f) of infection. (c, g) pDC depletion in DTR mice calculated compared to pDC percentages in WT mice on d42 (c) or d56 (g) of infection. (d, h) After SEA restimulation, or culture with medium alone, MLN cell-free supernatants were analyzed by ELISA for a schistosome egg-specific recall cytokine response. Medium alone values were subtracted from Ag-restimulated samples. Results are mean±SEM. Data from 3 experiments pooled (n=4–12 mice/ group) analyzed using a linear fixed effect model with pairwise comparison. *P<0.05, **P<0.01, ***P<0.001, P****<0.0001.