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. 2022 Feb 5;106(4):1557–1570. doi: 10.1007/s00253-022-11809-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — The new induction ratio IR* for comparison of ddPCR and RT-qPCR method. IR* — the new induction ratio. FL_Met- — the concentration of the full-length transcript in methionine starvation conditions. FL_Met+ — the concentration of full-length transcript in methionine presence conditions. T_Met- — the concentration of the terminated transcript in methionine starvation conditions. T_Met+ — the concentration of the terminated transcript in methionine presence conditions. P1_Met- — the concentration of the product amplified with the primers 1 in methionine starvation conditions. P1_Met+ — the concentration of the product amplified with the primers 1 in methionine presence conditions. P3_Met- — the concentration of the product amplified with the primers 3 in methionine starvation conditions. P3_Met+ — the concentration of the product amplified with the primers 3 in methionine presence conditions