Fig. 4.

Increased miR-9-5p regulates the expression of TFRC and GOT1 and relieves ferroptosis in vitro The bEnd.3 cells were transfected with miR-9-5p angomir, followed by serum stimulation. A The expression levels of NEAT1, miR-9-5p, TFRC, and GOT1 in control, model, and model + miR-9-5p angomir were tested by qRT-PCR (normalized to U6 or GAPDH). B The sequence for NEAT1 mutant. C The protein levels of TFRC and GOT1. D, E The immunofluorescence for TFRC and GOT1 (blue, DAPI; green, TFRC; red, GOT1). F The cell viability of bEnd.3 cells were evaluated by CCK-8 assay. G–K The levels of ROS, Fe ion (Fe²⁺ and Fe³⁺), GSH, GPX4, and MDA were assessed. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs control; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs model.