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. 2022 Jan 17;59(3):1954–1969. doi: 10.1007/s12035-022-02738-1

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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Enhanced NEAT1 promoted ferroptosis and increased the expression of TFRC and GOT1 in vitro NEAT1 was overexpressed in bEnd.3 cells. A The expression levels of NEAT1, miR-9-5p, TFRC, and GOT1 in Vector, WT NEAT1 (wild NEAT1 sequence), and MUT NEAT1 (site-directed mutant NEAT1) by qRT-PCR (normalized to U6 or GAPDH). B The protein levels of TFRC and GOT1 showed by western blotting. C, D The immunofluorescence for TFRC and GOT1 (blue, DAPI; green, TFRC; red, GOT1). E The cell viability of cells detected by CCK-8 assay. F–J The levels of ROS, Fe ion (Fe²⁺ and Fe³⁺), GSH, GPX4, and MDA. ^**P < 0.01, ^**P < 0.01, ^***P < 0.001 vs Vector; ^##P < 0.01, ^###P < 0.001 vs WT NEAT1.