Table 1.
Detailed biometric parameters findings and statistics.
| Parameter | CD | WD | F | P | ||
|---|---|---|---|---|---|---|
| VEH | NRG1 | VEH | NRG1 | |||
| Body weight (g) | 146.08 ± 2.30 | 146.88 ± 2.37 | 155.37 ± 1.88 | 157.03 ± 2.30 | Interaction: F (1, 66) = 0.03 | P = 0.85 |
| Treatment: F (1, 66) = 0.30 | P = 0.58 | |||||
| Diet: F (1, 66) = 19.20 | P < 0.0001 | |||||
| Caloric intake (kcal/d) | 52.84 ± 0.73 | 53.09 ± 0.96 | 63.39 ± 1.59 | 66.09 ± 1.04 | Interaction: F (1, 20) = 1.18 | P = 0.30 |
| Treatment: F (1, 20) = 1.71 | P = 0.21 | |||||
| Diet: F (1, 20) = 109.20 | P < 0.0001 | |||||
| PBG (mg/dl) | 128.08 ± 2.77 | 132.08 ± 3.08 | 144.92 ± 4.32 | 139.75 ± 4.04 | Interaction: F (1, 44) = 1.61 | P = 0.21 |
| Treatment: F (1, 44) = 0.026 | P = 0.87 | |||||
| Diet: F (1, 44) = 11.50 | P = 0.0015 | |||||
| Insulin (uIU/mL) | 38.92 ± 1.85 | 41.23 ± 4.82 | 34.23 ± 3.17 | 37.47 ± 3.87 | Interaction: F (1, 18) = 0.017 | P = 0.90 |
| Treatment: F (1, 18) = 0.45 | P = 0.45 | |||||
| Diet: F (1, 18) = 0.26 | P = 0.26 | |||||
| Corticosterone (ng/mL) | 18.01 ± 1.66 | 19.46 ± 3.79 | 23.40 ± 3.65 | 20.94 ± 3.06 | Interaction: F (1, 18) = 0.36 | P = 0.55 |
| Treatment: F (1, 18) = 0.02 | P = 0.87 | |||||
| Diet: F (1, 18) = 1.13 | P = 0.30 | |||||
| Leptin (pg/mL) | 597.57 ± 57.96 | 515.13 ± 22.62 | 640.33 ± 32.80 | 532.77 ± 57.07 | Interaction: F (1, 18) = 0.075 | P = 0.79 |
| Treatment: F (1, 18) = 4.27 | P = 0.05 | |||||
| Diet: F (1, 18) = 0.43 | P = 0.52 | |||||
| Triglycerides (mg/dL) | 118.35 ± 0.57 | 119.84 ± 0.93 | 119.48 ± 1.33 | 114.80 ± 3.13 | Interaction: F (1, 18) = 1.94 | P = 0.18 |
| Treatment: F (1, 18) = 0.32 | P = 0.32 | |||||
| Diet: F (1, 18) = 0.43 | P = 0.43 | |||||
| FGF-21 (ng/mL) | 0.72 + /- 0.07 | 0.59 + /- 0.07 | 0.91 + /- 0.13 | 1.12 + /- 0.11 | Interaction: F (1, 18) = 2.75 | P = 0.11 |
| Treatment: F (1, 18) = 0.70 | P = 0.70 | |||||
| Diet: F (1, 18) = 11.57 | P = 0.003 | |||||
The adolescent rats that consumed the WD exhibited an increase in body weight, food consumption, circulating FGF-21 levels, and post-prandial blood glucose levels relative to animals consuming the control diet at postnatal day 41. Three weeks of consuming the WD was not sufficient to alter plasma insulin, leptin, corticosterone, and triglyceride levels in adolescent rats. Exogenous NRG-1 administration did not have a significant effect on any of the biometric parameters measured. Bold denotes significant effects following two-way ANOVA. Sample numbers: Body weight: CD + VEH, n = 17; CD + NRG1, n = 17; WD + VEH, n = 18; WD + NRG1, n = 18. Caloric intake: CD + VEH, n = 6; CD + NRG1, n = 6; WD + VEH, n = 6; WD + NRG1, n = 6. PBG: CD + VEH, n = 12; CD + NRG1, n = 12; WD + VEH, n = 12; WD + NRG1, n = 12. Insulin, CORT, Leptin, TG, and FGF21: CD + VEH, n = 5; CD + NRG1, n = 5; WD + VEH, n = 6; WD + NRG1, n = 6.