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. 2022 Feb 26;13(2):190. doi: 10.1038/s41419-022-04623-0

Fig. 1. The circRNA circCYP24A1 is downregulated in RCC and associated with a favorable prognosis for patients with RCC.

Fig. 1

A The differential expression analysis showed that circRNAs were differentially expressed in RCC tumor and normal tissues (P < 0.05 and |log2FC| > 1.5). Notably, hsa_circ_0060927 was identified as the most highly differentially expressed circRNA. B The location and illustration of hsa_circ_0060927 (circCYP24A1). C Relative expression of circCYP24A1 in the RCC cell lines 786-O, 769-P, Caki-1, and A498 was evaluated and compared with the normal kidney proximal tubular cell line HK-2 using qRT-PCR. D qRT-PCR was performed to measure the relative expression of CYP24A1 and circCYP24A1 in 786-O and A498 cell lines treated with RNase R or vehicle control. E Relative expression of circular and linear CYP24A1 in 786-O and A498 cells incubated with actinomycin. F, G In situ hybridization was performed to assess the expression of circCYP24A1 in tumor and normal tissues from patients with RCC. H Kaplan–Meier analysis was conducted to measure the survival rate of patients with RCC presenting high or low circCYP24A1 expression. Data are presented as means ± SD. ***p < 0.001.