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. 2022 Feb 26;13(2):190. doi: 10.1038/s41419-022-04623-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Relative expression of circCYP24A1 was evaluated using qRT-PCR in 786-O and A498 cell lines transfected with LV-NC or LV-circCYP24A1. B Relative proliferation rates were evaluated in 786-O and A498 cell lines transfected with LV-NC or LV-circCYP24A1. The cell cycle distribution (C) and apoptosis rate (D) were evaluated in 786-O cell lines transfected with LV-NC or LV-circCYP24A1 using flow cytometry. Transwell assays (E) and wound-healing assays (F) were performed in 786-O and A498 cell lines transfected with LV-NC or LV-circCYP24A1. The bar corresponds to 300 μm in the wound-healing assay. G An EdU assay was performed to measure the proliferation of 786-O and A498 cell lines transfected with LV-NC or LV-circCYP24A1. The bar corresponds to 100 μm. Data are presented as means ± SD. ***p < 0.001.