Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 26;13(2):190. doi: 10.1038/s41419-022-04623-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — A The possible targets of miR-421 were predicted by four databases, including TargetScan, Encori, miRWalk, and KIRC Down. KIRC Down represents the downregulated genes in the differential gene expression analysis of TCGA-KIRC database. B Relative expression of THRB, PFKFB2, CMTM4, and PAPPA was measured using qRT-PCR in 786-O cells transfected with LV-NC or LV-circCYP24A1. C CMTM4 TPM expression in the tumor tissues compared with the normal tissues in TCGA-KIRC database. Overall survival (D) and disease-free survival (E) were measured using the Kaplan–Meier analysis. F Gene-set enrichment analysis of the MAPK signaling pathway in patients from TCGA-KIRC with high/low CMTM4 expression. G The predicted binding site for circCYP24A1 and miR-421 is illustrated. Mutant and wild-type CMTM4 constructs for dual-luciferase reporter assays were generated. H CMTM4 expression was detected using Western blotting in 786-O and A498 cells transfected with miR-421 mimic, miR-421 inhibitor, or its normal control. Data are presented as means ± SD. ***p < 0.001.