Figure 1.

Tissue-specific differences in transcript accumulation of V-ATPase subunit E in common ice plant. A, Northern-blot analysis of total RNA (15 μg per lane) isolated fom leaves (Lc) and roots (Rc) of 5-week-old unstressed plants and leaves (Ls) and roots (Rs) from 5-week-old plants stressed with 400 mm NaCl for 72 h. The northern blot was hybridized with a digoxigenin-labeled probe corresponding to the full-length AtpvE cDNA sequence. The lanes were loaded with aliquots from the same RNA preparation as used for the transcript quantitation shown in C. B, Amplification of a fragment of the coding region of AtpvE (TR) by RT-PCR. Linearity of amplification was tested in up to 24 amplification cycles in 5-week-old common ice plant. C, Quantitation of transcript amounts of V-ATPase subunit E in 3-, 5-, and 10-week-old common ice plant.