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. Author manuscript; available in PMC: 2022 Feb 27.
Published in final edited form as: Sci Immunol. 2019 Apr 19;4(34):eaaw4341. doi: 10.1126/sciimmunol.aaw4341

Fig. 5. Maternal Igs protect doubly deficient Nod2/Cybb mice from the development of colitis.

Fig. 5.

ELISA of Mucispirillum-specific IgA (A) and IgG (B) in the luminal content of fostered mice. Non-fostered Tac C57BL/6 (Tac B6) and Jax C57BL/6 (Jax B6) mice were included as controls. Data are mean ± SEM of at least six individual mice; data are from two independent experiments. **P < 0.01; ***P < 0.001;****P<0.0001byone-way ANOVA followed by Tukey’s multiple comparisons test. ELISA of Mucispirillum-specific IgA (C) and IgG (D) in the luminal content of Tac-DKO(n = 8) and Jax-DKO (n =6) mice. Results are mean ± SD; data are from two independent experiments. (E) Fecal Lcn-2 concentration in Tac-DKO (n = 8) and Jax-DKO (n = 6) mice. Data are mean ± SD; data are from two independent experiments. (C to E) Two-way ANOVA followed by Sidak’s multiple comparisons test. **P < 0.01; ***P < 0.001; ****P < 0.0001. (F) Mouse survival over time after fostering newborns DKO to either Jax JH−/− (DKO to Jax JH−/−, n = 7) or to Tac-exposed JH−/− mothers (DKO to Tac JH−/−, n = 15). Newborn Jax JH−/− (n = 7) and Tac JH−/− (n = 7) pups were included as controls. Data are from two independent experiments. Log-rank test. (G) Representative images (top) and H&E-stained colonic sections (bottom) from 9-day-old DKO fostered to Jax JH−/− or to Tac JH−/− mothers and Jax JH−/− and Tac JH−/− pups. Scale bars, 500 µm. (H) Histopathological scores of colonic tissues from (9-day-old) DKO fostered to Jax JH−/− (n = 6) or Tac JH−/− mothers (n = 15) and Jax JH −/− (n = 3) and Tac JH −/− mice (n = 3). Bars show median; data are from two independent experiments. *P < 0.05; **P < 0.01 by Kruskal-Wallis test followed by Dunn’s post test.