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. 2021 Jun 18;59(4):1570–1576. doi: 10.1007/s13197-021-05168-4

Table 1.

Identification of species with ITS-RFLP profile for isolated yeasts

Species identification Time Amplicon Endonuclease CfoI Endonuclease HaeIII Endonuclease HinfI Endonuclease DdeI
Saccharomyces cerevisiae Industrial inoculum 880 pb 360,320,130 320,240,180,130
Saccharomyces cerevisiae Time zero 880 pb 360,320,130 320,240,180,130
Hanseniaspora uvarum Time zero 750 pb 340, 190, 160 300, 180, 90, 80
Hanseniaspora opuntiae Time zero 750 pb 320, 310, 105 340, 190, 160 380, 180, 90, 80
Candida californica Time zero 450 pb 220, 105, 80, 70 290, 80, 60 240, 210  –
Hanseniaspora uvarum 24 h 750 pb 340, 190, 160 300, 180, 90, 80
Hanseniaspora opuntiae 24 h 750 pb 380, 170, 90, 80
Saccharomyces cerevisiae 24 h 880 pb 320,240,180,130
Saccharomyces cerevisiae 139 h 880 pb 320,240,180,130
Saccharomyces cerevisiae 188 h 880 pb 320,240,180,130