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. 2022 Feb 14;13:731500. doi: 10.3389/fimmu.2022.731500

Figure 2.

Figure 2

PHLDA1 attenuates LPS-initiated production of proinflammatory cytokines. RAW264.7 cells (A) and BMDM (B) were transfected with EV or PHLDA1 plasmid. PHLDA1 expression was measured at the protein and mRNA levels with Western blot (left panel) and/or RT-qPCR (right panel). RAW264.7 cells (C) and BMDM (D), which were transfected with EV or PHLDA1 plasmid, were treated with or without LPS (0.1 μg/ml) for 12 h. ELISA was performed to measure the production of proinflammatory cytokines (TNF-α, IL-6, and IL-1β). RAW264.7 cells (E) and BMDM (F) were transfected with Control siRNA and PHLDA1 siRNA1, and PHLDA1 expression was measured at the protein and mRNA levels with Western blot (left panel) and/or RT-qPCR (right panel). RAW264.7 cells (G) and BMDM (H), which were transfected with Control siRNA and PHLDA1 siRNA1, were treated with or without LPS (0.1 μg/ml) for 12 h. ELISA was performed to measure the production of proinflammatory cytokines (TNF-α, IL-6, and IL-1β). Data are shown as means ± SD of three independent experiments (NS means no significance, *P < 0.05; **P < 0.01; ***P < 0.001).