Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 28;7:60. doi: 10.1038/s41392-022-00881-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Intermediate stages of epithelial cells among the physiological, benign, and malignant stages. a Signature scores for cells from physiological, benign, and mixed clusters (9_Mix4) in P1. Columns represent cells (n = 2420) annotated with cell type and tissue. Colors represent Z scores. b Log-normalized expression levels of key marker genes of intestinal stem and base crypt cells (LGR5, SOX9, ASCL2, OLFM4). Red cycle indicates cells of cluster 9_Mix4 (top, P1, n = 73) and cluster 9_Mix2 (bottom, P2, n = 87). c Single-cell trajectory of 554 physiological epithelial cells, 73 adenoma-precursor cells (cluster 9_Mix4), and 1793 benign epithelial cells. The trajectory was constructed using monocle according to gene expression. Each dot represents a single cell, and colors represent cell types (left) and the pseudotime of trajectory (right). d Heatmap of differentially expressed genes among normal, adenoma precursor (cluster 9_Mix4), and adenoma epithelial cells. Genes related to carcinogenesis are highlighted. e Representative images of BMX and SH2D6 IHC staining of normal tissue (n = 20) and adenoma (n = 20). Black arrows indicate positively stained cells. Scale bar, 200 μm. f Venn diagrams show the overlap of upregulated genes in P1 epithelial mixed clusters (cluster 4, 5, and 9) and P2 epithelial mixed clusters (cluster 7 and 9)