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. Author manuscript; available in PMC: 2023 Mar 17.
Published in final edited form as: Cell Chem Biol. 2021 Aug 31;29(3):386–397.e9. doi: 10.1016/j.chembiol.2021.08.004

Figure 2. Design and biophysical characterization of the NSD3 PROTAC MS9715.

Figure 2.

(A) Chemical structures of the NSD3 PROTAC degrader MS9715 (top) and its analog control, MS9715N (bottom). See also Data S1.

(B-D) ITC titrations of BI-9321 (B), MS9715 (C) and MS9715N (D) into recombinant protein of the NSD3 PWWP1 domain. Binding affinity of BI-9321 (B), MS9715 (C) and MS9715N (D) to NSD3 was measured by ITC. The calculated values represent the mean ± SD from two independent experiments. First injection was removed from the fitting.