KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| FLAG® M2 antibody | Millipore Sigma | Cat # F1804; RRID:AB_262044 |
| VHL mouse polyclonal | Santa Cruz Biotechnology | Cat # SC135657; RRID:AB_2215955 |
| Ub mouse monoclonal (mAb) | Santa Cruz Biotechnology | Cat # SC8017; RRID:AB_628423 |
| NSD3 rabbit mAb | Cell Signaling Technology | Cat # 92056; RRID:AB_2800178 |
| HA tag rabbit mAb | Cell Signaling Technology | Cat # 3724; RRID:AB_1549585 |
| IFITM1 rabbit mAb | Cell Signaling Technology | Cat # 13126; RRID:AB_2798126 |
| Cleaved caspase-3 rabbit mAb | Cell Signaling Technology | Cat # 9661; RRID:AB_2341188 |
| Cleaved caspase-7 rabbit mAb | Cell Signaling Technology | Cat # 8438; RRID:AB_11178377 |
| α-Tubulin rabbit mAb | Cell Signaling Technology | Cat # 2144; RRID:AB_2210548 |
| GAPDH rabbit mAb | Cell Signaling Technology | Cat # 5174; RRID:AB_10622025 |
| cMyc rabbit mAb | Cell Signaling Technology | Cat # 9402; RRID:AB_2151827 |
| CBLB rabbit mAb | Cell Signaling Technology | Cat # 9498; RRID:AB_2797707 |
| Normal rabbit IgG | Cell Signaling Technology | Cat # 2729; RRID:AB_1031062 |
| Anti-mouse IgG, HRP-linked antibody | Cell Signaling Technology | Cat # 7076; RRID:AB_330924 |
| Anti-rabbit IgG, HRP-linked antibody | Cell Signaling Technology | Cat # 7074; RRID:AB_2099233 |
| Bacterial and Virus Strains | ||
| DH5a competent cells | Thermo Fisher Scientific | Cat# 18265017 |
| One Shot Stbl3 competent cells | Thermo Fisher Scientific | Cat# C737303 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| MS9715 | This study | NA |
| MS9715N | This study | NA |
| BI-9321 | (Bottcher et al., 2019) | NA |
| MLN4924 | Selleck Chemical | Cat # S7109 |
| MLN9708 | MedChemExpress | Cat # HY–10452 |
| Ac-VHL | This study | NA |
| Ac-VHL-me | This study | NA |
| Critical Commercial Assays | ||
| RNeasy Plus Mini Kit (250) | Qiagen | Cat # 74136 |
| Turbo DNA-free kit | Thermo Fisher | Cat # AM1907 |
| iSCRIPT cDNA Synthesis KIT | Biorad | Cat # 1708891 |
| iTaq Universal SYBR Green Supermix | Biorad | Cat # 1725125 |
| MycoAlert™ PLUS Mycoplasma Detection Kit | Lonza | Cat # LT27–286 |
| Lipofectamine 3000 Transfection Reagent | Thermo Fisher | Cat # L3000150 |
| NEBNext Ultra II RNA library Prep kit | New England Biolabs | Cat # E7770 |
| Nextseq 550 High Output Kit v2.5 | New England Biolabs | Cat # 20024906 |
| NEBNext Multiplex Oligos for Illumina | New England Biolabs | Cat # E7335S |
| Annexin V-FITC Apoptosis Detection Kit | BD Biosciences | Cat # 556570; RRID:AB_2869085 |
| SureBeads™ immunoprecipitation Kit with protein A and G conjugated magnetic beads | Biorad | Cat # 161–4833 |
| Deposited Data | ||
| Raw and analyzed mass spectrometry proteomics data | This study | PXD021901 |
| Raw and analyzed dataset of RNA-seq | This study | GEO: GSE158296 |
| Experimental Models: Cell Lines | ||
| K562 | ATCC | Cat # CCL–243; RRID:CVCL_0004 |
| RS4;11 | ATCC | Cat # CRL–1873; RRID:CVCL_0093 |
| MM1.S | ATCC | Cat # CRL–2974; RRID:CVCL_8792 |
| 293FT | Thermo Fisher | Cat # R70007 |
| MOLM-13 | DSMZ | Cat # ACC–554; RRID:CVCL_2119 |
| EOL-1 | DSMZ | Cat # ACC–386; RRID:CVCL_0258 |
| Oligonucleotides | ||
| RT-qPCR oligos and sgRNAs used | This study, Table S8 | NA |
| Recombinant DNA | ||
| pCDH-EF1 with HA-tagged cMyc | This study | NA |
| pLenti LRG-2.1_Neo with sgRNA of NSD3 (human) | This study | NA |
| Software and Algorithms | ||
| GraphPad Prism 8 | GraphPad | https://www.graphpad.com/ |
| FlowJo | FlowJo LLc | https://www.flowjo.com/ |
| ImageJ | ImageJ | https://imagej.nih.gov/ij/index.html |
| GSEA | (Subramanian et al., 2005) | https://software.broadinstitute.org/gsea/index.jsp |
| Perseus (V1.6.2.2) | Perseus | https://maxquant.net/perseus/ |
| Other | ||