Fig. 2.

The NLRP3 inflammasome is involved in early onset iAs-induced hepatic IR. (A–E) NLRP3 inflammasome-associated protein levels in control and iAs-treated livers. GAPDH served as a loading control (n = 3). (E–F) Serum IL-1β and IL-18 levels in control and iAs-treated mice (n = 6). (H–K) Total and phosphorylated AKT and GSK3β expression levels (H–J) and glucose uptake (K) in iAs-treated and MCC950 pretreated L-02 cells (n = 3). **P < 0.01 versus the control group, ^##P < 0.01 versus the iAs-treated group. The data represent the mean ± standard error of the mean (SEM), n = 3. Statistical significance was determined by Student's two-tailed t test (A–G) and one-way ANOVA (H–K)