Fig. 8.

Inhibition of m⁶A methylation attenuates iAs-induced hepatic IR. Before treatment with iAs, L-02 cells were transfected with siMETTL14 or siIGF2BP2. Cells were also stimulated with insulin for 10 min before protein isolation. (A–C) The effect of siMETTL14 on AKT and GSK3β phosphorylation levels in iAs-treated L-02 cells. (E–G) The effect of siIGF2BP2 on AKT and GSK3β phosphorylation levels in iAs-treated L-02 cells. (D and H) The effect of siMETTL14 or siIGF2BP2 on glucose uptake levels in iAs-treated L-02 cells. *P < 0.05 and **P < 0.01 versus the control group, ^#P < 0.05 and ^##P < 0.01 versus the iAs-treated group. The data represent the mean ± standard error of the mean (SEM), n = 3. One-way ANOVA was used to determine statistical significance