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. 2022 Feb 14;13:814942. doi: 10.3389/fphar.2022.814942

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Copyright © 2022 Ma, Zheng, Su, Zhang, Liu, Li, Zhang, Gong and Kou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EDA mitigates cerebral endothelial apoptosis stimulated by MCAO/R in mice. (A) Brain-frozen sections were stained with TUNEL (green) and CD31 was used as a marker for endothelial cells; the nuclei were stained with DAPI (blue) (scale bar = 20 μm). (B) Quantitative analyses of apoptotic cells in endothelial cells. (C) Western blot analysis for the expression of Bax and Bcl-2 in brain tissues. (D) Western blot analysis for the expression of cleaved caspase-3 in brain tissues. All data are presented as the means ± SEM, n = 6. ^# p < .05, ^## p < .01, ^### p < .001, vs. Sham group, *p < .05, **p < .01, ***p < .001, vs. MCAO/R group.