Table 1.
Primers used in the present study
| Marker | ID in CS RefSeq v1.1 | Primer sequence 5'–3' (Forward) | Primer sequence 5'–3' (Reverse) | Size (bp) | Enzyme | Function |
|---|---|---|---|---|---|---|
| pkw4974 | TraesCS7A02G497400 | GCACTCCAGGTGTCGCTCAG | ACCATTTCTCGCCGCTGTTC | 619 | HaeIII | Fine mapping |
| pkw4982 | TraesCS7A02G498200 | GTATGTGAAATAGAAAATGGGCAAC | CATAAGATTGCTGCCAAAGAACT | 944 | MfeI | Fine mapping |
| pkw4984 | TraesCS7A02G498400 | CCATTTGCTCCCACGAACA | CCCCATCAAGCCACTCTAT | 607 | MboII | Fine mapping |
| Pkw4990 | TraesCS7A02G499000 | TGAAAGGGAAGGTGAAGGA | AGGTGGAGGTTAAGGCGAG | 970 | BsaJI | Fine mapping |
| pkw4995 | TraesCS7A02G499500 | CTCAGAACACGGCTTCAACA | GATCACATGGACCTTCATCG | 900 | SspI | Fine mapping |
| Tm5F3R4 | TraesCS7A02G499600 | TGGAGAAAGTGGACAAGAT | GCTGCTCTATCTTCGGTTG | 971 | PvuII | Fine mapping |
| TM5TF3R3 | TraesCS7A02G499600 | GGATTTAGGGTTTCGGGGA | CCAACTACCACCACGGACG | 1137 | – | Fine mapping |
| pkw4997 | TraesCS7A02G499700 | TATGCCCAAAAGGAGTAGG | TACATCCTGTAGGACAAAACTG | 709 | AccI | Fine mapping |
| pkw4999 | TraesCS7A02G499900 | TGTCTACTGCATGAAGTTCAACC | AGCGGTCTCATTGACGGAA | 799 | AatII | Fine mapping |
| pkw5001 | TraesCS7A02G500100 | CGGTGTAGCATACCATTTCG | TTTCTTGTAGAGCGGGAGC | 1448 | – | Fine mapping |
| pkw5003 | TraesCS7A02G500300 | CTGTTGCTCAACGCCCATCTC | GATCACGTCGGGCATGAACTTATA | 675 | SmaI | Fine mapping |
| pkw5009 | TraesCS7A02G500900 | TCTTGCTGTTGCTTGGCTGTC | TGTCCCGCCTGTTGTTCCT | 1205 | SphI | Fine mapping |
| TM5TF2R2 | TraesCS7A02G499600 | GCACTGAGACTCCTCGGTGATGT | CACTCATATTACCCCCTTCCTTACC | 673 | – | MAS |
| A120F6R6 | TraesCS7A02G499600 | AAGAACTTGCTGCCGGACAT | AATCTTGTACCTTGAAAATCTGTCG | 108 | – | Expression analysis |
| HL‐F61R60 | TraesCS7A02G499600 | GTTGCAGAGTTTTCGGGTTTACC | GGCTTTCCGATGAAGTCATAGAA | 109 | – | Expression analysis |
| 4997QF2R2 | TraesCS7A02G499700 | CCAAAAGGAGTAGGAGTACA | ACGCATCATATCAAAGAAAC | 260 | – | Semi‐quantitative PCR |
| 4998QF5R5 | TraesCS7A02G499800 | CATTCTAAAGGTGTGATGGATTA | ATTGGCCTTTCTGAGGTTGG | 272 | ‐ | Semi‐quantitative PCR |
| TM5AF6R8 | TraesCS7A02G499600 | CTAGACAATTACATCAAGGTATA | GGGTATCAATCCAATCATCTCAATA | 1688 | Sequencing | |
| TM5AF4R4 | TraesCS7A02G499600 | GGTGTCCTCTCTCTGTAAACTGG | ATCTATTTGCTCGTCTCGTAACATA | 649 | Sequencing | |
| cfa2049 | – | TAATTTGATTGGGTCGGAGC | CGTGTCGATGGTCTCCTTG | – | Introgression | |
| wmc405 | – | GTGCGGAAAGAGACGAGGTT | TATGTCCACGTTGGCAGAGG | – | Introgression | |
| cfd68 | – | TTTGCAGCATCACACGTTTT | AAAATTGTATCCCCCGTGGT | – | Introgression | |
| gwm260 | – | GCCCCCTTGCACAAATC | CGCAGCTACAGGAGGCC | – | Introgression | |
| barc108 | – | GCGGGTCGTTTCCTGGAAATTCATCTAA | GCGAAATGATTGGCGTTACACCTGTTG | – | Introgression | |
| barc121 | – | ACTGATCAGCAATGTCAACTGAA | CCGGTGTCTTTCCTAACGCTATG | – | Introgression | |
| wmc790 | – | AATTAAGATAGACCGTCCATATCATCCA | CGACAACGTACGCGCC | – | Introgression |