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. 2021 Nov 16;20(3):468–484. doi: 10.1111/pbi.13729

Figure 8.

Figure 8

Overexpression of OsCSLD4 enhances rice salt tolerance by up‐regulating ABA synthesis. (a) Phenotype of OsCSLD4 overexpression rice seedlings treated with salt stress in soil. Two‐week‐old rice seedlings grown in soil were treated with 150 mm NaCl for 7 days with another 7‐day recovery. (b) Survival rate of OsCSLD4 overexpression rice seedlings treated with 150 mm NaCl for 7 days with another 7‐day recovery. (c) ABA content in 2‐week‐old OsCSLD4 overexpression rice seedlings. (d) Expression level of ABA synthesis and responsive genes in 2‐week‐old OsCSLD4 overexpression transgenic rice seedlings. The expression of OsActin1 was used as the internal control. The gene expression levels in transgenic rice seedlings were presented as relative to the levels in the wild type (Kitaake). Kitaake: wild type. OEx: independent OsCSLD4 overexpression lines. ‘x’ indicates the numbering of different OsCSLD4 overexpression transgenic lines. The figure presents the average of at least three repeated experiments. Bars indicate the SE (±) from three repeated experiments. Asterisks represent significant difference as evaluated by t‐test (*P < 0.05 and **P < 0.01).