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. 2022 Feb 14;12:748594. doi: 10.3389/fmicb.2021.748594

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Copyright © 2022 Wang, Wu, Du, Mei, Luo, Liu, Saleemi, Zhang, Chang, Mehmood and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histopathological analysis of colon segments. (A,B) Staining of colon tissues of mice with hematoxylin–eosin (H&E), original magnifications of × 100 (A) and × 200 (B). (C) Goblet cells observed with Alcian blue/periodic acid–Schiff (AB/PAS) staining. (D) Determination of the histological scores for the control (C), dextran sodium sulfate (DSS) model (CD), and Tibetan tea + DSS (TD) groups. (E) Sections immunostained with mouse anti-myeloperoxidase (MPO) antibody in each group, with the positive cells staining brown. The positive rate of MPO cells (the number of positive cells/total cell number × 100) was calculated using HALO. Lowercase letters a–c indicate significant differences in all treatment groups (p < 0.05).