Table 1.

Selective pressure analysis for the branch-site models.

Receptor	Model	np	Ln L	Estimates of parameters					Model compared	LRT P-value	Positive sites a
TLR5	Model A	28	-10165.09641	Site class	0	1	2a	2b	Model A vs.Model A null	0.001143784	530 N 0.978*
				f	0.60473	0.33234	0.04061	0.02232
				ω0	0.10087	1	0.10087	1
				ω1	0.10087	1	9.07143	9.07143
	Model A null	27	-10170.38592	1							Not Allowed
TLR8	Model A	28	-16158.517734	Site class	0	1	2a	2b	Model A vs.Model A null	0.000231386	823 A 0.965*
				f	0.57462	0.40766	0.01037	0.00735
				ω0	0.07029	1.00000	0.07029	1.00000
				ω1	0.07029	1.00000	998.99874	998.99874
	Model A null	27	-16165.296401	1							Not Allowed
TLR13	Model A	20	-7100.573648	Site class	0	1	2a	2b	Model A vs.Model A null	0.001826836
				f	0.64259	0.32938	0.01853	0.00950
				ω0	0.06914	1.00000	0.06914	1.00000
				ω1	0.06914	1.00000	14.37178	14.37178
	Model A null	19	-7105.431583	1							Not Allowed
TLR22	Model A	32	-17590.839397	Site class	0	1	2a	2b	Model A vs.Model A null	0.000001954	344 S 0.962*,627 M 0.960*
				f	0.49499	0.44812	0.02986	0.02703
				ω0	0.13232	1.00000	0.13232	1.00000
				ω1	0.13232	1.00000	12.14526	12.14526
	Model A null	31	-17602.159433	1							Not Allowed
TLR23	Model A	16	-9143.957160	Site class	0	1	2a	2b	Model A vs.Model A null	0.009946886	Not significant sites
				f	0.56141	0.41365	0.01436	0.01058
				ω0	0.11399	1.00000	0.11399	1.00000
				ω1	0.11399	1.00000	19.63740	19.63740
	Model A null	15	-9147.279352	1							Not Allowed
Gya_TLR21_2	Model A	28	-14048.172839	Site class	0	1	2a	2b	Model A vs.Model A null	0.000003564	25 D 0.952*, 427 D 0.973*, 513 Q 0.956*, 552 R 0.998**, 577 D 0.973*
				f	0.55406	0.39020	0.03270	0.02303			615 M 0.976*,653 R 0.963*,770 D 0.975*,809 V 0.972*,815 S 0.974*
				ω0	0.06857	1.00000	0.06857	1.00000
				ω1	0.06857	1.00000	53.35043	53.35043
	Model A null	27	-14058.915882	1							Not Allowed

^aOnly PSS with significant posterior probabilities are indicated. Asterisks indicate posterior probabilities P≥95%(*) and p>99%(**).

LRT Statistics (2ΔL) and p-values for the branch-site models on the ancestral Nototheniidae branch for Tlr5, Tlr8, Tlr13, Tlr22, Tlr23, and G. acuticeps Tlr21 duplicate (Tlr21_2).

"w" symbol is Omega which is calculated by Codeml. This value indicates the classes of sites analyzed (emphasis on estimated).