Figure 3.

Programmable targeting of SVV RNA by Cas13d. The CasRx-del-NLS plasmid cotransfected with (A) control crRNA plasmid or (B) targeted crRNA#1 plasmid into HEK293T cells. At 24 hpi, the cells were infected with SVV (at an MOI of .3). Thirty-six hours post-infection, SVV VP2 was detected by IFA with an anti-VP2 monoclonal antibody and Alexa Fluor 568-conjugated goat antibodies. CasRx expression was indicated by EGFP, and nuclei were labeled with DAPI. (C) Manders’ colocalization coefficients was analyzed, six random selected images were analyzed by ImageJ. ^***p < 0.001.