Figure 1.

Detection of isoamylase complexes on glycogen-containing zymograms. Native crude extracts (100 μg of protein) were loaded on a rabbit liver glycogen containing zymogram (see “Materials and Methods”). C. reinhardtii isoamylase complexes appears as white bands after incubation at the top of the gel. The isoamylase defective mutants carrying the sta7-1::ARG7 mutation show no band on this zymogram. Wild-type strains always display three distinct bands of similar intensity, whereas sta8-1::ARG7 strains display only one lower white band on over 50 meiotic segregants (27 mutant strains and 26 wild type).