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. 2001 Apr;125(4):1723–1731. doi: 10.1104/pp.125.4.1723

Figure 5.

Figure 5

DTT sensitivity of C. reinhardtii isoamylases. The fresh crude extracts from synchronized cultures were used to assay isoamylase activities in wild-type and sta8 strains using bovine liver glycogen as substrate (see “Materials and Methods”) at various DTT concentrations. Results are displayed as nanomoles of maltotriose formed by hour and per milligram of protein. The x axis represents the DTT concentrations used in the incubation buffer. Means (●, ▪, ○ for wild-type [330], sta8-1::ARG7 [BafV13], sta7-4::ARG7 [Bafj6] strains, respectively) and sd from three distinct measures were calculated for each DTT concentration.