Figure 7.

Immunogold localization of legumin in endosperm of transformed wheat 16 dap. Ib, Inclusion body; p, protein body. A, Discrete, dark-stained regions (arrows) of the inclusion bodies immunolabel heavily (inset shows gold particles) for legumin. Bar = 1 μm. B, Angular legumin-containing crystals are heavily immunolabeled and project from the inclusion bodies into the vacuole. Bar = 500 nm. C, Crystal with 9-nm lattice structure decorated with 15-nm immunogold label for legumin. Bar = 100 nm. D, Transformed wheat not expressing legumin. Background immunogold labeling over protein bodies and inclusion bodies is low and similar to that in control untransformed wheat. Bar = 2 μm.