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. 2022 Feb 14;13:815968. doi: 10.3389/fendo.2022.815968

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Copyright © 2022 Liao, Qi, Yun, Qiao and Pang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DHEA impaired mouse follicular growth and steroidogenesis in vitro. (A) Representative micrograph of mouse follicles cultured in vitro. (B) Follicle diameters in control and DHEA group, N = 9. (C) Estradiol levels in the supernatant of control and DHEA-treated follicles, N = 3. (D) mRNA expression levels of Cyp17a1, Cyp19a1, and Amh in follicles cultured in vitro, N = 3. (E) mRNA expression levels of Lss, Acss2, Ldlr, Insig1, Srebf1, Fasn, Fads2, and Hmgcr in follicles cultured in vitro, N = 3. Scale bar: 100 μm. Data were analyzed by two-tailed Student’s t-test (B–E). All data are presented as the Mean ± SEM. *P < 0.05, **P < 0.01.