Table 2.
Primer Sequence used in quantitative real-time PCR analysis.
| Target genes | Primer sequence |
|---|---|
| 18S (mouse) | Forward 5’-GAAACGGCTACCACATCCAAGG-3’ |
| Reverse 5’-GCCCTCCAATGGATCCTCGTTA-3’ | |
| Cyp17a1 (mouse) | Forward 5’-GCCCAAGTCAAAGACACCTAAT-3’ |
| Reverse 5’-GTACCCAGGCGAAGAGAATAGA-3’ | |
| Cyp19a1 (mouse) | Forward 5’-ATGTTCTTGGAAATGCTGAACCC-3’ |
| Reverse 5’-AGGACCTGGTATTGAAGACGAG-3’ | |
| Amh (mouse) | Forward 5’-CCACACCTCTCTCCACTGGTA-3’ |
| Reverse 5’-GGCACAAAGGTTCAGGGGG-3’ | |
| Gdf9 (mouse) | Forward 5’-TCTTAGTAGCCTTAGCTCTCAGG-3’ |
| Reverse 5’-TGTCAGTCCCATCTACAGGCA-3’ | |
| Bmp15 (mouse) | Forward 5’-TCCTTGCTGACGACCCTACAT-3’ |
| Reverse 5’-TACCTCAGGGGATAGCCTTGG-3’ | |
| Has2 (mouse) | Forward 5’-TGTGAGAGGTTTCTATGTGTCCT-3’ |
| Reverse 5’-ACCGTACAGTCCAAATGAGAAGT-3’ | |
| Ptx3 (mouse) | Forward 5’-CCTGCGATCCTGCTTTGTG-3’ |
| Reverse 5’-GGTGGGATGAAGTCCATTGTC-3’ | |
| Adamts1 (mouse) | Forward 5’-CATAACAATGCTGCTATGTGCG-3’ |
| Reverse 5’-TGTCCGGCTGCAACTTCAG-3’ | |
| Tnfaip6 (mouse) | Forward 5’-GGGATTCAAGAACGGGATCTTT-3’ |
| Reverse 5’-TCAAATTCACATACGGCCTTGG-3’ | |
| Hmgcr (mouse) | Forward 5’-AGCTTGCCCGAATTGTATGTG-3’ |
| Reverse 5’-TCTGTTGTGAACCATGTGACTTC-3’ | |
| Fads2 (mouse) | Forward 5’-GATGGCTGCAACATGACTATGG-3’ |
| Reverse 5’-GCTGAGGCACCCTTTAAGTGG-3’ | |
| Fasn (mouse) | Forward 5’-GGAGGTGGTGATAGCCGGTAT-3’ |
| Reverse 5’-TGGGTAATCCATAGAGCCCAG-3’ | |
| Srebf1 (mouse) | Forward 5’-GCAGCCACCATCTAGCCTG-3’ |
| Reverse 5’-CAGCAGTGAGTCTGCCTTGAT-3’ | |
| Insig1 (mouse) | Forward 5’-CACGACCACGTCTGGAACTAT-3’ |
| Reverse 5’-TGAGAAGAGCACTAGGCTCCG-3’ | |
| Ldlr (mouse) | Forward 5’-AGTGGCCCCGAATCATTGAC-3’ |
| Reverse 5’-CTAACTAAACACCAGACAGAGGC-3’ | |
| Acss2 (mouse) | Forward 5’-AAACACGCTCAGGGAAAATCA-3’ |
| Reverse 5’-ACCGTAGATGTATCCCCCAGG-3’ | |
| Lss (mouse) | Forward 5’-TCGTGGGGGACCCTATAAAAC-3’ |
| Reverse 5’-CGTCCTCCGCTTGATAATAAGTC-3’ | |
| ACTB (human) | forward 5’-GAGCACAGAGCCTCGCCTTT-3’ |
| reverse 5’-TCATCATCCATGGTGAGCTGG-3’ | |
| HMGCR (human) | Forward 5’-TGATTGACCTTTCCAGAGCAAG-3’ |
| Reverse 5’-CTAAAATTGCCATTCCACGAGC-3’ | |
| FASN (human) | Forward 5’-TGATTGACCTTTCCAGAGCAAG-3’ |
| Reverse 5’-CTAAAATTGCCATTCCACGAGC-3’ | |
| SREBF1 (human) | Forward 5’-CGGAACCATCTTGGCAACAGT-3’ |
| Reverse 5’-CGCTTCTCAATGGCGTTGT-3’ | |
| SCD (human) | Forward 5’-TTCCTACCTGCAAGTTCTACACC-3’ |
| Reverse 5’-CCGAGCTTTGTAAGAGCGGT-3’ | |
| INSIG1 (human) | Forward 5’-GCCTACTGTACCCCTGTATCG-3’ |
| Reverse 5’-TGGTTAATGCCAACAAAAACTGC-3’ | |
| LDLR (human) | Forward 5’-ACGGCGTCTCTTCCTATGACA-3’ |
| Reverse 5’-CCCTTGGTATCCGCAACAGA-3’ | |
| LSS (human) | Forward 5’-GTACGAGCCCGGAACATTCTT-3’ |
| Reverse 5’-CGGCGTAGCAGTAGCTCAT-3’ | |
| SCD5 (human) | Forward 5’-TGCGACGCCAAGGAAGAAAT-3’ |
| Reverse 5’-CCTCCAGACGATGTTCTGCC-3’ | |
| FADS2 (human) | Forward 5’-GACCACGGCAAGAACTCAAAG-3’ |
| Reverse 5’-GAGGGTAGGAATCCAGCCATT-3’ | |
| ACSS2 (human) | Forward 5’-AAAGGAGCAACTACCAACATCTG-3’ |
| Reverse 5’-GCTGAACTGACACACTTGGAC-3’ |