Table 2.

Effects of dietary AFB₁ and XL on relative mRNA expression of IL-1β, IL-6, and CYP3A4 in the spleen and liver of broiler chickens.¹²³

Groups		Spleen		Liver
		IL-6		IL-1β		IL-6		CYP3A4
		21 d	42 d	21 d	42 d	21 d	42 d	21 d	42 d
Control		1.00b	1.00	1.00	1.00b	1.00	1.00	1.00	1.00
XL		1.28b	0.54	1.21	1.40b	0.38	0.14	0.34	0.34
AFB1		8.43a	1.24	1.09	3.52	0.67	2.30	0.67	1.01
XL+AFB1		0.80b	2.38	0.86	1.24b	0.46	0.69	0.27	0.37
SEM4		0.748	0.274	0.198	0.294	0.088	0.235	0.091	0.125
The main effect
AFB1	-	1.14	0.77	1.10	1.20	0.69	0.53	0.69	0.65
	+	4.62	2.38	1.47	2.38	0.56	1.50	0.56	0.69
XL	-	4.71	1.12	1.55	2.34	0.83	1.71	0.83	1.00
	+	1.04	1.46	1.03	1.31	0.42	0.41	0.42	0.34
P-value5
AFB1		<0.001	0.052	0.323	0.020	0.417	0.014	0.417	0.864
XL		<0.001	0.50	0.176	0.058	0.014	0.002	0.014	0.007
AFB1$\times$ XL		<0.001	0.127	0.064	0.009	0.203	0.291	0.203	0.889

^a-bMeans with different superscripts in the same column differ significantly (P < 0.05).

¹Each value represents the mean values of 6 pens of 10 animals each (n = 6).

²AFB₁, aflatoxin B₁; BWG, body weight gain; FI, feed intake; FCR, feed conversion ratio.

³− represents without AFB₁ or XL supplementation; + represents supplemented with AFB₁ or XL.

⁴SEM, standard error of the mean.

⁵P-values represent the main effect of AFB₁ exposure, the main effect of the dietary XL, and the interaction between AFB₁ exposure and XL treatments.