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. Author manuscript; available in PMC: 2023 Apr 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Biol Lipids. 2022 Jan 18;1867(4):159094. doi: 10.1016/j.bbalip.2021.159094

Figure 2. crd1Δ increases ethanol production in response to heat stress in galactose.

Figure 2.

Cells were grown at 30°C in SC-glucose medium to the mid-logarithmic phase, washed twice with sterile water, resuspended in fresh (A) SC-galactose (n=4) or (B) SC-glucose (n=3) medium, and diluted to an A550 of 0.5. After shaking incubation for two or five h at 39°C, supernatant from 1 mL of culture was collected by centrifugation. Ethanol contained in the medium was measured as described in Materials and Methods. Data shown are mean ± S.D. Unpaired t test; **, p≤0.01, ***, p≤0.001. (C) WT, crd1Δ, adhxΔ (x=1, 2, 3, 4, 5), and crd1ΔadhxΔ cells were pre-cultured in SC-glucose medium to the stationary phase and diluted to an A550 of 0.5. 5 μL aliquots of a series of 10-fold dilutions were spotted on SC-glucose or SC-galactose plates and incubated at 39 °C for three days.