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. 2022 Feb 14;9:820105. doi: 10.3389/fcell.2021.820105

FIGURE 4.

FIGURE 4

iPSC-MN lacking NFL differentiate into functional motor neurons with reduced miniature excitatory postsynaptic current amplitude. Motor neuron electrophysiological characteristics were measured by whole cell patch-clamp during the seventh week of differentiation. (A) Example traces of spontaneous action potentials. (B) Frequency of spontaneous action potentials in current-clamp (CTR vs. KO1 multiple-comparison p = .0106). (C) Resting membrane potential. (D) Example traces of action potentials evoked by depolarization and a scheme of the depolarizing current injected. (E) Action potential amplitude. (F) Action potential half-width. (G) After-hyperpolarizing potential amplitude. (H) Action potential threshold (CTR vs. KO1 p = .0322). (I) Rheobase (CTR vs. KO1 p < .0001). (J). Evoked action potential frequency (p = .02, two-way repeated measures ANOVA). (K) Example traces of miniature excitatory post synaptic currents (mEPSCs) and averaged mEPSCs shown with the expanded time scale. (L). Amplitude of mEPSC is reduced in iPSC-MNs lacking NFL (PT/KO1/KO2 vs. CTR p < .0170). (M) Frequency of mEPSC in iPSC-MNs. Data shown as individual measurements/neurons and mean with SD. *p < .05, **p < .0001. One-way ANOVA followed with Dunnett’s multiple comparison post hoc test vs. CTR and two-way ANOVA followed with Tukey’s multiple comparison post hoc test for J. # indicates abnormal karyotype.