Figure 4.

Clinical chemistry analysis of urine and plasma of mice backcrossed 10 generations to the B6 background (B6-Lama5^E884G/E884G). (a) Urinalysis showed proteinuria at 25 weeks. (b–d) There is significant hypoalbuminemia from 15 weeks (b), urea is slightly more elevated in homozygous (HOM) plasma at 25 weeks (c), but there is no difference in creatinine levels (d). (e) Creatinine clearance, an indicator of glomerular filtration rate (GFR), is normal in mutant mice at all ages tested. (f–h) Nonetheless, B6-Lama5^E884G/E884G mice exhibited a nephrotic phenotype with hypercholesterolemia in addition to hypoalbuminemia at 25 weeks. The values shown are means ± SEM. Two-way analysis of variance with Bonferroni post hoc test was used. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001. HDL, high-density lipoprotein; LDL, low-density lipoprotein; WT, wild type.