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. 2022 Feb;192(2):226–238. doi: 10.1016/j.ajpath.2021.10.011

Figure 3.

Figure 3

Dedicator of cytokinesis 2 (DOCK2) deficiency protects mice from chronic high-fat and high-fructose (HFHF) diet–induced lung inflammation/injury. A: Body weight of DOCK2 knockout (DOCK2−/−) mice fed a normal chow diet or an HFHF diet for 20 weeks. B and C: Representative hematoxylin and eosin staining (B) and Masson’s staining (C) of lung tissues from wild-type (WT) or DOCK2−/− mice fed an HFHF diet for 20 weeks. D: Lung tissues from WT or DOCK2−/− mice fed an HFHF diet for 20 weeks were collected. Two representative samples for each group were loaded to detect collagen (Col-1) protein expression by Western blotting. Actin is a loading control. E and F: Immunofluorescence (IF) shows increased CD68 (green, E) and monocyte chemoattractant protein-1 (MCP-1) (red, F) expression in lung tissues from WT or DOCK2−/− mice fed an HFHF diet for 20 weeks. DAPI stained the nuclei (blue). The black boxes indicate the areas enlarged for view. Quantification of expression levels in E and F from 5 fields per slide per mouse and 3 mice per group. n = 6 to 8 (A). ∗P < 0.05 versus WT HFHF diet group. Scale bar = 100 μm.