Figure EV3. Transient expression of NEK6 in HEK293 cells does not affect the cell cycle (related to Fig 6E).

Control experiments with HEK293 cell expressing the indicated NEK6 and NEK6‐KD constructs. No significant differences in the proportions of cells in the different phases of the cell cycle can be observed, suggesting that changes in ERα ‐dependent gene activation cannot be attributed to cell cycle alterations.

1. Cells were transfected with the indicated amounts of NEK6 or NEK6‐KD plasmids for 24 h and stained with and Hoechst 33258. The cellular DNA content was measured by flow cytometry and the percentage of cells in G1, S, and G2 was calculated using ModFit LT software.
2. Representative cell cycle profiles generated by the ModFit LT software for the indicated transfection experiments.