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. 2021 Mar 11;107(3):680–689. doi: 10.3324/haematol.2020.260562

Figure 4.

Figure 4.

Loss of ZRSR1 exacerbates retention of U12-type introns in ZRSR2-deficient murine hematopoietic cells. (A) Distribution of DMSI values for retention of U12-type introns in Lin−Kit+ bone marrow (BM) cells lacking either ZRSR1 (sh1 or sh10) or ZRSR2 or both compared to control cells. (B) Number of U12-type introns retained (P<0.05; Fisher's exact test) in various pair-wise comparisons of Lin−Kit+ BM cells deficient in either one or both ZRSR proteins. (C) Normalized expression of representative U12-type introns in Lin−Kit+ BM cells determined using quantitative real-time polymerase chain reaction. Expression of U12-type introns was measured relative to expression of flanking exons. Data are from at least three replicates and represented as mean ± standard error of the mean. P-values for each group compared to the ‘ZRSR2 WT; con sh’ group are depicted in the plot. Statistical difference between the Zrsr2/Zrsr1-double deficient and Zrsr2 KO cells are shown below the graph. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; ns: not significant; MSI: mis-splicing index: Difference in MSI values (DMSI) was calculated as DMSI=MSIknockout−MSIwildtype.