Skip to main content
. 2021 Mar 11;107(3):680–689. doi: 10.3324/haematol.2020.260562

Figure 5.

Figure 5.

Aberrant retention of U12-type introns in human MAPK9 and MAPK14 genes impacts their protein expression. (A and B) Normalized expression of U12-type introns of MAPK9 (A) and MAPK14 (B) in TF1 and K562 cells stably expressing short hairpin RNA (shRNA) targeting human ZRSR2. The expression of U12-type introns was measured relative to the expression of flanking exons using quantitative polymerase chain reaction (qPCR). Data are from five PCR experiments and represented as mean ± standard error of the mean. *P<0.05, **P<0.01, ***P<0.001, ns: not significant. (C) Protein levels of human MAPK9 and MAPK14 in TF1 and K562 cells transduced with shRNA targeting ZRSR2.