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. 2022 Feb 14;10:821875. doi: 10.3389/fcell.2022.821875

FIGURE 3.

FIGURE 3

Overexpression of MUC1 leads to increased phosphorylation of JNK and c-Myc and knockdown of MUC1 reduces phosphorylation of JNK and c-Myc. (A) Western blot expression of phosphorylation of JNK and c-Myc compared to total JNK and total c-Myc in MiaPaca2 vs MiaPaca2. MUC1 cells in response to 10 ng/ml of TGF-β at 10 min. (B) Western blot expression of phosphorylation of JNK and c-Myc compared to total JNK and total c-Myc in HPAFII cells treated with control siRNA vs MUC1 siRNA in response to 10 ng/ml of TGF-β at 10 min. (C) Densitometric analysis of fold change of expressions of pJNK/Total JNK and p-c-Myc/Total c-Myc normalized to endogenous β-actin is presented in MiaPaca2 cells. (D) Densitometric analysis of fold change of expressions of pJNK/Total JNK and p-c-Myc/Total c-Myc normalized to endogenous β-actin is presented in HPAFII cells. (E) Knockdown efficiency of MUC1 in HPAFII after 72 h of siRNA treatment. Data are presented as means ± SEM of n = 3; Unpaired Student’s t-test and one-way ANOVA were used to analyze the differences between treatment groups. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.