Effect of mock and IAV infection (FLU) and treatment with 2-DG, DCA, or ROT from 1–5 d.p.i. on: (A) Carotid arterial O2 saturation (SaO2; n=9, 26, 5, 8, 10, 16, 6, and 8 per group, from 16 independent mock or IAV infections); (B) Heart rate (n=9, 26, 5, 8, 10, 16, 6, and 8 per group, from 16 independent mock or IAV infections); (C) Lung water content (wet:dry weight ratio; n=6, 9, 5, 7, 5, 10, 8, and 7 per group, from 16 independent mock or IAV infections); and (D) Viral replication (n=5/group, from 2 independent IAV infections per group). Data in box represent first quartile, median, and third quartile for each experimental group. Whiskers indicate highest and lowest sample values within the group. *: P<0.05, **: P<0.005, #: P<0.001, vs. untreated mock-infected mice. §: P<0.001, vs. IAV-infected mice.