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. 2022 Feb 28;41:79. doi: 10.1186/s13046-022-02288-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — ZNF498 directly interacts with p53 and represses p53 transcriptional activity by inhibiting p53 Ser46 phosphorylation. A, B Reciprocal co-IP of exogenous (A) and endogenous (B) ZNF498 and p53 in HepG2 cells. The indicated whole cell lysate and immunoprecipitates were analyzed by Western blotting. rIgG: rabbit IgG, mIgG: mouse IgG. C Direct interaction between ZNF498 and p53 as revealed by GST pulldown assays. D Map of the region of ZNF498 that interacts with p53. Cell lysates from HCT116 p53^−/− cells transfected with Myc-tagged deletion mutants of ZNF498 and Flag-tagged p53 were immunoprecipitated with an anti-Myc antibody before they were subjected to Western blotting. E Map of the region of p53 that interacts with ZNF498. Cell lysates from HCT116 p53^−/− cells transfected with Flag-tagged ZNF498 and Myc-tagged deletion mutants of p53 were immunoprecipitated with an anti-Myc antibody before they were subjected to Western blotting. FL: full length