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. 2022 Feb 28;21:62. doi: 10.1186/s12943-022-01502-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — circCAMSAP1 is highly expressed in NPC. A The expression of circCAMSAP1 was measured in 29 NPC tissues and 12 non-cancerous NPE tissue samples by RT-qPCR. β-actin was used as an internal reference. NPE, nasopharyngeal epithelium; NPC, nasopharyngeal carcinoma. ***, p < 0.001. B Representative images of circCAMSAP1 in 82 NPC tissues and 29 adjacent NPE tissues by in situ hybridization. × 200, scale bar = 50 μm; × 400, scale bar = 20 μm. The statistical data was shown in the right panel. ****, p < 0.0001. C The stability of circCAMSAP1 was detected in three RNase R-treated NPC cells by RT-qPCR. CAMSAP1 mRNA was used as a negative control. ***, p < 0.001; ****, p < 0.0001. D The stability of circCAMSAP1 was examined in NPC cells after treating with actinomycin D for 0, 6, 12 and 24 h. The relative RNA levels of circCAMSAP1 and CAMSAP1 mRNA in NPC cells were measured by RT-qPCR. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. E Intracellular localization of circCAMSAP1 (red) in three NPC cells, as determined by fluorescence in situ hybridization using a digoxigenin-labeled circCAMSAP1 probe. Nuclei were stained with DAPI (blue). Scale bar = 20 μm. F The cellular localization of circCAMSAP1 was examined in three NPC cells by nuclear and cytoplasmic separation test. GAPDH and U6 were used as positive controls for cytoplasm and nucleus, respectively