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. 2022 Feb 28;21:62. doi: 10.1186/s12943-022-01502-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — circCAMSAP1 stabilizes the expression of SERPINH1 by binding to its 3’UTR. A Western blotting analysis of SERPINH1 expression in three NPC cell lines after overexpression and knockdown of circCAMSAP1. B RT-qPCR analysis of SERPINH1 expression in three NPC cell lines after overexpression and knockdown of circCAMSAP1. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. C The luciferase reporter gene activity was analyzed in three NPC cell lines after overexpression or knockdown of circCAMSAP1. The wild-type (WT) or mutant (MT) of the SERPINH1 3’UTR were constructed and transfected into NPC cells. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. D The circRIP assay was used to detect the binding of circCAMSAP1 to the 3’UTR of SERPINH1 mRNA. **, p < 0.01; ****, p < 0.0001. E The stability of SERPINH1 mRNA was measured in NPC cells after overexpression or knockdown of circCAMSAP1. Cells were treated with actinomycin D for 0, 60, 120 min and RT-qPCR was used to measure the expression of SERPINH1 mRNA. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001