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. 2022 Feb 28;22:214. doi: 10.1186/s12885-022-09318-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — A Volcano plot of VIP scores from OPLS-DA model. The green crosses represent the metabolites with VIP>1 and the grey crosses represent the metabolites with VIP ≤ 1. B Volcano plot with the univariate statistical test (−ln P) and the magnitude of the change (log2FC) of metabolites. Red points represent the metabolites with P-value < 0.05 and FC>0. Blue points represent the metabolites with P-value < 0.05 and FC < 0. Grey points represent the metabolites with P-value>0.05. C Venn diagram integrating results from volcano-plots of OPLS-DA model and univariate statistical test